Scientist Using Microscope

Custom Peptide Synthesis

  1. Personalized Peptide Synthesis

    • Quantum chemical peptide design

    • Solid phase peptide synthesis

    • Special amino acid incorporation

    • Purity >95%

  2. Characterization

    • HPLC-UV  - primary tool

    • HPLC-MS

      • For fragments that can only be followed by MS detection

      • Fragmentation patterns will be predicted based on MS masses

    • NMR

      • High resolution structure characterization

    • CD spectroscopy

      • Secondary structure characterization

Microscope

Pre-clinical Drug Testing and
Diagnostics Laboratory

SERVICES

  • Gene expression analysis

  • Protein expression analysis

  • Histology

  • Immunohistochemistry

  • Immunofluorescent staining

  • 2D cell culture models

  • 3D organoid culture models

  • Signal transduction pathways (Ca2+ and cAMP signalling)

  • In vitro disease modelling

  • In vivo disease modelling

  • In vivo drug distribution analysis

FACILITIES​

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Microscopy​

​The Microscopy Facility provides access to different cutting edge light and electron microscopy imaging modalities. We cover the whole process of sample preparation, imaging and image analysis of fixed as well as live samples.

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Olympus widefield
microscope systems

Oxford Nanoimager super resolution microscope

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Zeiss LSM 880
microscope systems

Zeiss Sigma 300
scanning electron microscope

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Animal Facility

The Animal Facility houses mice (including knockout strains) and rats adhering to the strictest animal welfare obligations.

 

In vivo assessment programs are available for

  • in vivo toxicology,

  • drug distribution and

  • disease modelling studies.

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Molecular Biology

The Molecular Biology Facility provides access to different molecular biology techniques to study gene and protein expression. We cover the whole process of sample preparation, experiments and data analysis. These tools can be used in combination with other services (such as in vitro, or in vivo treatments).

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Quantitative Real-time PCR

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Western blott

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Cell Cultures

The Cell Culture Facility provides access to conventional 2D cell line (HEK293, Hela, etc) as well as novel state of the art 3D organoid cell culture models.  

 

In the recent years organoid cultures (OCs) derived from tissue specific Leucine-rich repeat-containing G-protein coupled receptor 5 positive (Lgr5+) adult stem cells emerged as novel models of organ development and disease. By maintaining the activity of Wnt/β-Catenin signal transduction cascade – a key drivers of most types of tissue stem cells - OCs can be grown in vitro for long-term in 3D extracellular matrix based hydrogels; whereas, epithelial cells in the culture maintain the original cellular diversity and organization of the organ of origin.

 

Currently, OCs can be established from a wide range of organs (such as colon, esophagus, stomach, liver and pancreas, lung, etc).

 

Patient-derived organoid cultures posses a huge translational potential as they allow to utilize human tissue (normal, or diseased) to study disease development or screen drugs.